|
|
|
|
|
| Pinto,S.C.C.; Almeida,D.S.; Alves,M.B.R.; Florez-Rodriguez,S.A.; Abreu Júnior,G.S.; Alves,N. Britto e; Celeghini,E.C.C.; Laskoki,L.M.; Souza,F.A.. |
| ABSTRACT The aim of this study was to evaluate the addition of vitamin C, reduced glutathione and the association thereof to the bovine semen cryopreservation extender. The ejaculate from nine bulls were divided into four fractions, each corresponding to a treatment, namely: control group-semen diluted with Tris-yolk extender; vitamin C group-semen diluted in Tris-yolk extender supplemented with vitamin C (2.5mmol/mL); glutathione group-semen diluted in Tris-yolk extender supplemented with reduced glutathione (2.5mmol/mL) and associated group-semen diluted in Tris-yolk extender supplemented with vitamin C (1.25mmol/mL) and reduced glutathione (1.25mmol/mL). Afterwards, the semen was packed into French straws and submitted to cryopreservation using... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reactive oxygen species; Sperm membranes; Spermatozoa. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100009 |
| |
|
|
| Celeghini,E.C.C.; Gregory,L.; Pinheiro,E.S.; Piva,F.M.; Carneiro,P.A.B.; Parapinski-Santos,B.; Bianchi,M.; Benesi,F.J.. |
| O objetivo deste artigo é relatar um caso de orquiepididimite associado com infecção por Salmonella enterica subespécie diarizonae em carneiro da raça Santa Inês de quatro anos de idade, vasectomizado. Ao exame clínico reprodutivo, o animal mostrou aumento severo do conteúdo escrotal, sendo o testículo direito maior do que o esquerdo e a cauda do epidídimo direita maior do que a esquerda. A consistência testicular, avaliada em escala de 1 a 5, foi 5 para o testículo direito e 2,5 para o esquerdo; o órgão apresentava-se muito sensível ao toque. Na ultrassonografia foram observadas estruturas anecoicas/hipoecoicas circulares na cauda do epidídimo, sugestivas de abscessos; alguns pontos hiperecogênicos no parênquima testicular, sugerindo lesões de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epididimite; Orquite; Patologia; Infecção; Ovino. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100021 |
| |
|
|
| Celeghini,E.C.C.; Nascimento,J.; Raphael,C.F.; Andrade,A.F.C.; Arruda,R.P.. |
| In this experiment, it was defined a protocol of fluorescent probes combination: propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA), and JC-1. For this purpose, four ejaculates from three different rams (n=12), all showing motility >80% and abnormal morphology <10%, were diluted in TALP medium and split into two aliquots. One of the aliquots was flash frozen and thawed in three continuous cycles, to induce damage in cellular membranes and to disturb mitochondrial function. Three treatments were prepared with the following fixed ratios of fresh semen:flash frozen semen: 0:100 (T0), 50:50 (T50), and 100:0 (T100). Samples were stained in the proposal protocol and evaluated by epifluorescence microscopy.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovine; Semen; Epifluorescence microscopy; Sperm membranes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300006 |
| |
|
|
|
